Dr. Yasmeen Taj

PhD Theme/Topic: Detection of High-risk Human Papilloma Virus (HPV 16 and 18) with genotyping DNA in urine samples for abnormal Papanicolaou test and cervical cancer.

Supervisor: Dr. Yasmeen Taj: Professor
Contact #: 0092-3032094439
Email: yasmin.bumdc@bahria.edu.pk 
Campus/School/Dept: BUHSCK
RAC Approved Supervisor for Research Areas: Pathology

Supervisory Record:
PhD Produced: NA 
PhD Enrolled: NA
MPhil Produced: 06
MPhil Enrolled: 02

Topic Brief Description:

Human Papilloma Virus (HPV), particularly high-risk genotypes 16 and 18, is a major cause of cervical cancer. This cross-sectional study aims to detect and genotype HPV 16 and 18 DNA in urine samples of women aged 20–45 years with abnormal Papanicolaou (Pap) smear results and cervical cancer. Cervical cytology will be classified according to the revised Bethesda system, and HPV DNA will be detected using PCR-based methods, followed by genotyping of positive samples. The study evaluates the association between HPV genotypes and the severity of cervical lesions. Findings from this study may support urine-based HPV testing as a simple, non-invasive, and cost-effective screening tool for early detection of cervical cancer in resource-limited settings.

Research Objectives/Deliverables:

1. To evaluate the morphological pattern of cervical cytology (Pap smear) and characterize the lesions according to revised Bethesda system.
2. To detect HPV DNA in urine of patients with abnormal Pap smear.

Research Questions: 

1. Can high-risk Human Papilloma Virus (HPV 16 and 18) DNA be reliably detected and genotyped in urine samples of women with abnormal Papanicolaou test results and cervical cancer?
2. What is the distribution of cervical cytological abnormalities (ASCUS, LSIL, HSIL) according to the revised Bethesda system among women aged 20–45 years?
3. Is there a significant association between abnormal Pap smear categories (ASCUS, LSIL, HSIL) and the presence of high-risk HPV DNA in urine samples?
4. Are HPV 16 and/or 18 genotypes significantly associated with high-grade squamous intraepithelial lesions (HSIL) and cervical cancer?

Candidate’s Eligibility Profile:

1. The applicant must have an MPhil/Equivalent degree in Microbiology with CGPA > 3.0. Besides, applicants must have a strong background in microbiology, genetics, and molecular techniques.
2. Experience with hands on for SPSS, Mega X and Prism software is advantageous. Candidates should thrive in an international environment and have excellent communication skills to actively contribute to team research efforts.
3. Proficiency in spoken and written English is essential. We value independence and responsibility while promoting teamwork and collaboration among colleagues.